Objective: The purpose of our study was to determine the median values of the triple test screening parameters in pregnancy in our region.

Material and Methods: In this study we evaluated the serum alpha feto protein (AFP), unconjugated estriol (uE₃) and human chorionic gonadotrophin (β-HCG) values of 2834 pregnant women who admitted to Biochemistry Laboratory for the prenatal triple test in 2007.

Results: The median values were calculated for each parameter between the gestational 15-20 weeks. We found that the AFP median values were significantly low, uE₃ median values were significantly higher compared to previously used values of 15th-20th weeks (p< 0.05). β-HCG median values were high except of 18th weeks.

Conclusion: We believed that using the regional median values in triple test, performance of the screening tests can be improved, prenatal risks can be calculated more accurately, and the need for use of invasive diagnostic procedures can be decreased.