Ana Sayfa | Amaç ve Kapsam | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | İletişim  
2010, Cilt 8, Sayı 1, Sayfa(lar) 009-014
[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
Comparison of Immunofluorometric and Immunuturbidimetric Methods in CRP Determination
Yasemin Üstündağ, Kağan Huysal, Ayşe Ulusoy Karaca, Teoman Çınar, Serpil Sancar, Müberra Akdoğan
Şevket Yılmaz Eğitim ve Araştırma Hastanesi, Biyokimya ve Mikrobiyoloji Laboratuarı, Bursa
Keywords: i-chroma, PETIA, Dimension RCRP, method comparison, POCT

Objective: C-reactive protein (CRP) is one of acute phase respondents that has been used to monitor infection and inflammation episodes. In our hospitals' emergency laboratory, CRP analysis is done with a point of care testing device (i-chroma). In our central biochemistry laboratory, same analysis is done with an autoanalyser (Dimension RXL). The aim of this study was to study the performance of immunofluorometric i-Chroma micro-CRP method against the immunoturbidimetric Dade-Behring RCRP method as the reference.

Materials and Methods: Control sera were used to detect between-run and between-day precision. Samples from 81 subjects were collected and CRP levels were determined (double-blind, parallel manner) using both systems.

Results: The imprecision of intra- and the inter-assay CVs (coefficient of variation) of assay systems were CVs< %6 in the range of 16.7 mg/L and 35.1 mg/L. Accuracy was evaluated in the selected fresh sera with CRP levels between 2.5-.227 mg/L. Correlation coefficient was r=0.993 and the regression equation was y=1.091x-1.92.

Conclusion: The i-Chroma micro-CRP assay system is comparable to well-known fully automated Dimension RCRP assay and is suitable for point-of-care testing (POCT) in detection and quantification of CRP.


[ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ]
Ana Sayfa | Amaç ve Kapsam | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | İletişim