2021, Cilt 19, Sayı 2, Sayfa(lar) 139-144 |
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Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement |
Bilal İlanbey1, Kenan Güçlü2 |
1Kırşehir Ahi Evran Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya, Kırşehir, Türkiye 2Kırşehir Eğitim ve Araştırma Hastanesi, Biyokimya Laboratuvarı, Kırşehir, Türkiye |
Keywords: Hemolysis; interference; preanalytical error; troponin |
Aim: Measurement of cardiac troponins is of great importance in the diagnosis and follow-up of acute
myocardial infarction. The diagnostic specificity of myocardial infarction has increased with algorithms
including serial troponin measurements. It is important that the measurements in the troponin cut-off
values used to evaluate the risk of myocardial infarction in algorithms are reliable. In this study, it was
aimed to evaluate the effect of hemolysis on clinical decision concentrations of the high sensitive
cardiac troponin I (hs cTnI) test.
Material and Methods: Two pools were created from serum samples with the 99th percentile upper
reference value (17.5 ng/L) of hs cTnI and the high-risk cut-off value (50 ng/L) as specified in the
European Society of Cardiology 2020 manual. Hemolysate was spiked in both pools with a hemolysis
index of 100 to 1000.
The hs cTnI levels in hemolysate-added sera were measured in Beckman Coulter UniCel Dxl 800
immunoassay device. Levels of hemolysis were calculated as percentage change.
Results: Due to hemolysis, hs cTnI values decreased in varying proportions from 3.8% to 20.4% at 99th
percentile concentration; values decreased from 4.3% to 20.0% in high-risk cut-off concentration.
Conclusions: In hemolyzed samples, hs cTnI values decrease, this decrease is not considered clinically
significant until high hemolysis values. However, it should be kept in mind that a decrease that may
occur even in a low hemolysis may lead to erroneous evaluations in clinical decision concentrations.
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