2017, Cilt 15, Sayı 3, Sayfa(lar) 129-133 |
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Interference of Variant Hemoglobin in Measurement of HBA1C by Ion-Exchange HPLC |
Fatih Yay, Fatma Uçar, Ali Yalçındağ, Lütfüye Tuba Hancı, Esra Çetin, İsmail Temel |
Sağlık Bilimleri Üniversitesi Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Tıbbi Biyokimya, ANKARA, Türkiye |
Keywords: Variant Hemoglobin; HbD-Los Angeles; HbA1c; Interference |
Background: It has been established that hemoglobinopathies and chemically modified derivatives of
hemoglobin, interfered with some HbA1c assay methods either positively or negatively. In this study we
present a case with a low HbA1c value as measured by ion-exchange HPLC method.
Case presentation: A 55 year old patient admitted to policlinic of Internal Medicine and the result of
HbA1c was 2.1%, measured by ion-exchange HPLC method in our laboratory. Unusual peaks and
warning about variant hemoglobin were detected in the result chromatogram. Following on suspicion of
hemoglobin variant sent to referral laboratory, was determined result of HbD-Los Angeles 84.4%, HbA
6.9%, HbA2 3.9%, HbF 4.8% (Trinity Biotech variant analyzer).
Discussion: Variant hemoglobin may cause negative interference in methods such as ion-exchange
HPLC, while immunological methods do not cause significant interference in the measurement.
Therefore, in the presence of clinically incompatible HbA1c levels, hemoglobin variants should be
investigated.
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