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2016, Cilt 14, Sayı 2, Sayfa(lar) 073-078
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Comparison of HPLC methods in HbA1c determination
Özlem Uğurlu Demirezen, Nuriye Uzuncan, Sibel Bilgili, Murat Akşit, Alperen Halil İhtiyar, Özge Esenlik, Giray Bozkaya
İzmir Bozyaka Eğitim ve Araştırma Hastanesi, Tıbbi Biyokimya, İzmir, Türkiye
Keywords: HbA1c protein; HPLC; Methodological studies

Objective: Method comparison experiments are based on comparison of samples results analyzed by the new test method and previously accepted method. In this study is aimed to compare reversedphase cation exchange chromatography with boronate affinity chromatography method which is currently used in HbA1c measurements in our laboratory.

Materials and Methods: One hundred and thirty one patient samples which were sent to biochemistry laboratory for HbA1c measurement were included in this study. HbA1c measurements were made in Adams HA-8180V (Arkray, KDK, Shiga, Japan- reversed-phase cation exchange chromatography) and Premier Hb9210 (Trinity Biotech, Ireland-boronate affinity chromatography) HPLC instruments.Method comparison studies were made according to National Committee for Clinical Laboratory Standarts (NCCLS) EP-9 A2 protocol which was published by Clinical Laboratory Standarts Institute.

Results: Results of 131 patient samples were recorded to SPSS 21.0 for method comparison. The correlation between two methods was statistically significant (r= 0.980, p< 0.05) and the regression equation was found as (y= 0.27+0.95x).

Conclusion: HbA1c measurements with two different methods were determined to be compatible,accurate and reliable. Besides,we thought that it will be appropiate to consider other factors such as speed and cost when selecting the methods for routine use.


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