2016, Cilt 14, Sayı 1, Sayfa(lar) 046-050 |
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Comparison of vitamin D levels measured by HPLC and Immunoassay |
Müge Bekmez1, Özkan Alataş2 |
1Iğdır Devlet Hastanesi, Tıbbi Biyokimya, Iğdır, Türkiye 2Eskişehir Osmangazi Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Eskişehir, Türkiye |
Keywords: Vitamin D, immunoassay, method comparison |
Objective: However, the analytical and standardization problems have continued, vitamin D status is
usually assessed by measuring the plasma concentration of 25-hydroxyvitamin D [25(OH)D]. The aim of
this study was to compared vitamin D levels of 50 healthy volunteers measured by High Performance
Liquid Chromatography and Immunoassay methods.
Material and Methods: 50 healthy volunteers were involved. Vitamin D was determined both in Agilent
1100 which is used High Performance Liquid Chromatography kits and Roche Diagnostics E170 in
plasma specimens. The results were evaluated statistically.
Results: The median value was 16.50 μg/l for High Performance Liquid Chromatography assay and
13.48 μg/l for immunoassay method. A positive correlation was found between methods by Spearman's
correlation test (p<0.001, r:0.78). The within-run and between run coefficient of variations for
immunoassay method were 3.6 and 3.7 respectively. When we compared the test stabilities of
specimens according to the storage condition and time, we found deviations from the initial value.
Conclusion: We conclude that the electrochemiluminescence assay provides the benefits such as
improving laboratory workflow and efficiency, being precise, providing greater convenience. We
determined that the samples were assayed in the same day.
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