Objectives: There are not enough studies based on laboratory results regarding the prevalence of Substance Use Disorders in our country, The purpose of this retrospective study is; to determine the distribution of the substances used by dependent individuals with treatment desire according to age, gender and laboratory data and to share the results of this wide sampling.

Methods: In 9402 urine samples between 2015-2020; analysis of alcohol, amphetamine, 3,4- methylenedioxy-N-methylamfetamine, cannabis, cocaine and morphine were performed by ultraperformance liquid chromotography coupled with tandem mass spectrometry (LC/ MS/ MS).

Results: Reportable levels of items were determined in 3792 samples of 2274 individuals. General mean age was calculated as 31.14 ± 9.32. The addictive substance users were found mostly between age 25-29 for men (23.8%) and 20-24 (20%) for women. It was determined that female between the ages of 14-19 use more substances (14.8%) than male who are in the same age range (%5.14) (p <0.001). The most used item ranking was; cannabis, alcohol, cocaine (40.2%, 24.1%, 19.6%) and cannabis users were observed to increase each year. In samples with more than one substance; there were 2 (21%) and 3-6 (5%) different substances. Those who used multiple substances also chose more cannabis, alcohol and cocaine.

Conclusion: The results of this study showed differences between; Turkey and the European Union drug reports and with some regional studies. There are some differences in the particularly the most widely used material ranking but a similarity in the increasing of cannabis use. Some similarities were found in the profile of substance users by age ranges and genders. Diagnosis, treatment and follow-up will be made by the more widespread use of laboratory data from large epidemiological studies, Turkey will help to achieve more concrete results in general.