HbA1c measurement was performed by the high-performance liquid chromatography (HPLC) method, and the result was measured as 3.5% (14.75 mmol/mol). Since the HbA1c result was inconsistent with the other clinical values of the patient, the same sample was run in the variant mode of the instrument and Hb variant warning was received. The HbD variant was detected in the analysis of the sample and the HbA1c result was reported as 5.1% (32 mmol/mol). In the presence of a low HbA1c value that is not suitable for the patient's clinic, the sample should be re-examined considering the presence of Hb variant.