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2021, Cilt 19, Sayı 1, Sayfa(lar) 001-004
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Effect of Negative Pressure in the Tube on Hemolysis
Arzu Kösem, Canan Toğçuoğlu, Sevilay Sezer, Turan Turhan
Ankara Şehir Hastanesi, Biyokimya Laboratuvarı, Ankara, Türkiye
Keywords: Hemolysis; index; serum

Purpose: A parameter associated with the result of a measurement that characterises the dispersion of the values that could reasonably be attributed to the measurand is defined as measurement uncertainty (U). Measurement uncertainty is very important for the clinician to provide medical judgement, diagnosis and treatment. The aim of this study was to determine the measurement uncertainty of fT4, fT3 and TSH parameters in our laboratory.

Material and Methods: 49 patients who admitted to our hospital's blood collection unit were included in the study. Blood samples were randomly collected into two BD VacutainerTM 5 ml serum seperator tubes by venipuncture from each patient. One of the tubes was marked to ensure standardization in 2 ml of blood draw. Up to this marked area, 2 ml of blood was collected from the patients and the other blood tube was used as recommended by the manufacturer. After the samples were centrifuged at 1500 g for 15 minutes, LDH, AST, K tests were analyzed on the Beckman Coulter AU 5800. Results were evaluated with paired-t test.

Results: LDH, AST, K results were significantly higher in low volume tubes than normal volume filled tubes(p <0.001).

Conclusion: In our study, there was no visible or index-calculated hemolysis in all tubes. However, AST, LDH, K levels were found to be significantly higher in low volume tubes. According to these results, of vacuum inside the tube concluded causes hemolysis.


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