2021, Cilt 19, Sayı 1, Sayfa(lar) 001-004 |
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Effect of Negative Pressure in the Tube on Hemolysis |
Arzu Kösem, Canan Toğçuoğlu, Sevilay Sezer, Turan Turhan |
Ankara Şehir Hastanesi, Biyokimya Laboratuvarı, Ankara, Türkiye |
Keywords: Hemolysis; index; serum |
Purpose: A parameter associated with the result of a measurement that characterises the dispersion of
the values that could reasonably be attributed to the measurand is defined as measurement uncertainty
(U). Measurement uncertainty is very important for the clinician to provide medical judgement,
diagnosis and treatment. The aim of this study was to determine the measurement uncertainty of fT4,
fT3 and TSH parameters in our laboratory.
Material and Methods: 49 patients who admitted to our hospital's blood collection unit were included
in the study. Blood samples were randomly collected into two BD VacutainerTM 5 ml serum seperator
tubes by venipuncture from each patient. One of the tubes was marked to ensure standardization in 2
ml of blood draw. Up to this marked area, 2 ml of blood was collected from the patients and the other
blood tube was used as recommended by the manufacturer. After the samples were centrifuged at 1500
g for 15 minutes, LDH, AST, K tests were analyzed on the Beckman Coulter AU 5800. Results were
evaluated with paired-t test.
Results: LDH, AST, K results were significantly higher in low volume tubes than normal volume filled
tubes(p <0.001).
Conclusion: In our study, there was no visible or index-calculated hemolysis in all tubes. However, AST,
LDH, K levels were found to be significantly higher in low volume tubes. According to these results, of
vacuum inside the tube concluded causes hemolysis.
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