2016, Cilt 14, Sayı 2, Sayfa(lar) 100-111 |
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Comparison of Two Different Electrophoresis Systems in Transferrin Isoform Analysis |
Öznur Bilen1, Özlem Gürsoy Çalan2, Zekiye Sultan Altun3, Pınar Akan2, Canan Çoker2 |
1Muğla Sıtkı Koçman Üniversitesi Eğitim Araştırma Hastanesi, Tıbbi Biyokimya, MUĞLA, Türkiye 2Dokuz Eylül Üniversitesi Tıp Fakültesi, TIıbbi Biyokimya Anabilim Dalı, İZMİR, Türkiye 3Dokuz Eylül Üniversitesi Tıp Fakültesi, Onkoloji Enstitüsü Temel Onkoloji Anabilim Dalı, İZMİR, Türkiye |
Keywords: transferrin; isoelectric focusing ; glycosylation |
Objective: Transferrin isoform analysis is performed for diagnosis of congenital or acquired defects in
protein glycosylation. The reference method for transferrin isoform analysis is isoelectric focusing
electrophoresis. The objective of this study has been to perform transferrin isoform analysis using two
different electrophoresis systems in our laboratory and to compare the two systems.
Materials and Methods: The isoelectric focusing electrophoresis of transferrin isoforms was
performed by "Multiphor II" ve "PhastSystem". The analytical performance was evaluated using a
commercially available quality control material at two different levels. The two systems were compared
with regression analysis with blood samples obtained from 40 patients.
Results: In isoelectric focusing method by "Multiphor II" system the coefficients of variation (CV) were
determined to be < 3.5 % for within run (n=7) and < 7 % for between run (n=20) imprecision
considering di- and tetrasialotransferrin isoforms in two levels of control material. The same values for
"PhastSystem" were < 7% (n=8) and < 6% (n=10) respectively. In IEF with “PhastSystem”,
disialotransferrin isoforms were determined to be 9.69% and 9.27% higher while tetrasialotransferrin
isoforms were determined to be 1.33% and 2.15% higher compared to “Multiphor II” at low and high
levels of control material respectively. In the regression analysis (n=40) coefficients of correlation for dive
tetrasialotransferrin isoforms were determined to be 0,85 ve 0,64 respectively.
Conclusion: Transferrin isoform analysis with isoelectric focusing electrophoresis can be performed
precisely with similar accuracy with both “Multiphor II” and “PhastSystem”. In routine laboratory practice
“PhastSystem” may be preferred due to its speed.
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