Ana Sayfa | Amaç ve Kapsam | Dergi Hakkında | İçindekiler | Arşiv | Yayın Arama | Yazarlara Bilgi | İletişim  
2012, Cilt 10, Sayı 3, Sayfa(lar)
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Evaluation of Histopathologic Examination of The Liver and Serum Liver Function Tests in Long Term Acrylamide Given Rats
Fatma Hümeyra Yerlikaya1, Aysun Toker1, Yeşim Yener2, Hatice Toy3
1Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Biyokimya Anabilim Dalı, Konya
2Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Biyoloji Eğitimi Bölümü, Konya
3Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Patoloji Anabilim Dalı, Konya
Keywords: Acrylamide; liver function tests; malondialdehyde

Objective: Acryla mide is formed during cooking, frying and burning at high temperatures, of many commonly consumed foods. After determining the adverse effects of acrylamide on health, increased the research numbers on this topic. The aim of this study was to investigate serum liver function tests, liver tissue malondialdehyde (MDA) levels and histopathologic examination of the liver in long term acrylamide (AA) given rats, compared to control rats.

Materials and Methods: In total, 25 male and 25 female wistar rats were involved in this experiment. AA was administered to the treatment groups at 2 and 5 mg/kg./day via drinking water for 90 days. At the end of the experiment, the serum samples were analyzed for total cholesterol, triglycerides, total protein, albumin, AST, ALT, LDH, and the liver tissues were analyzed for MDA levels and histopathological examination.

Results: The levels of serum AST, ALT, LDH, triglycerides and the levels of liver MDA of 5 mg/kg/day AA-treated male rats were found to be significantly higher than those of the controls (p<0.01 for LDH, and p<0.05 for AST, ALT, triglycerides and MDA). Similarly, serum LDH (p<0.05) levels were significantly increased in 2 mg/kg/day AA-treated male rats compared to control rats. Additionally, serum AST (p<0.05), LDH (p<0.01) and liver tissue MDA levels of 5 mg/kg/day AA-treated female rats were significantly increased. Also, our findings showed that there was more liver damage (single cell necrosis, steatosis and nuclear pleomorphism) in terms of both quality and quantity in both sexes in 5 mg/kg/gün AA-treated rats compared to 2 mg/kg/gün AA-treated rats.

Conclusion: Our findings showed that AA consumption has the harmfull effects on the biochemical functions and histological structure of liver. We believe that AA consumption in the diet should be reduced.


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