Objective: The discrepancy between chemical and microscopic urine examination makes it demanding to interpret the results. We aimed to evaluate the laboratory personnels’ effect on the consistency between chemical and microscopic urine examination results.

Material and Methods: The urinalysis results of a tertiary hospital’s core and supervised laboratory between 2019 and 2020 were evaluated retrospectively. The consistency between chemical and microscopic urine examination and laboratory personnel’s effect on this compliance was examined by correlation and measurement of agreement analysis.

Results: The core laboratory had higher consistency in erythrocyte count and blood, leukocyte count and leukocyte esterase. Accordingly, we determined that a yeast cell count of >30 significantly reduced the conformity between blood and erythrocyte counts in the supervised laboratory. The correlation between erythrocyte count and blood increased when controlling for yeast cell count for supervised and core laboratory.

Conclusion: Laboratory personnel significantly affect the consistency between automated urinalysis chemical and microscopic examination results. For the reliability of the results, it is essential to reduce the unnecessary workload of laboratory personnel and increase the time they will spend on specific tests.