2021, Cilt 19, Sayı 1, Sayfa(lar) 050-055 |
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Comparison of the Lifotronic H9 and Primus Ultra2 HPLC systems for the detection of HbA2 |
Mehmet Erten, Yeter Çakmak |
Halk Sağlığı Laboratuvarı, Tıbbi Biyokimya, Malatya, Türkiye |
Keywords: Beta Thalassemia; Hemoglobinopathy; HbA2; HPLC; Lifotronic H9; Method Comparison |
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Aim: Beta thalassemias are common autosomal recessive inherited hemoglobinopathy worldwide.
Hemoglobin A2 levels are the most important parameter used to determine beta-thalassemia carriers. One
of the most commonly used methods for HbA2 measurement is high-performance liquid chromatography
(HPLC). We aimed to compare the HbA2 levels of Lifotronic H9 and Primus Ultra2 HPLC systems.
Material and Methods: A total of 51 individuals, 19 females and 32 males were included in our study.
The compatibility of the results was demonstrated by the Bland-Altman plot and Passing-Bablok
regression analysis.
Results: The geometric mean ratio of values was 0.95 (95% limits of agreement 0.6 to 1.49) in the
Bland-Altman plot. 95% confidence intervals were observed as -1.03 - 1.30 (intercept) and 0.55-1.33
(slope) in Passing-Bablok regression analysis.
Conclusion: We reported first time measuring compatibility of Lifotronic H9 HPLC system in terms of
HbA2 levels by using Primus Ultra2. |
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Abstract
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