2021, Cilt 19, Sayı 3, Sayfa(lar) 200-211 |
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The Relationship Between DAS-28 Score and Oxidative Stress Markers in Rheumatoid Arthritis |
Duygu Eryavuz Onmaz1, Abdullah Sivrikaya1, Sedat Abuşoğlu1, Sema Yılmaz2, Ali Ünlü1 |
1Selçuk Üniversitesi, Tıp Fakültesi Biyokimya Anabilim Dalı, Konya, Türkiye 2Selçuk Üniversitesi, Tıp Fakültesi Romatoloji Anabilim Dalı, Konya, Türkiye |
Keywords: Rheumatoid Arthritis; Oxidative Stress; Inflammation; Ischemia Modified Albumin; Prolidase |
Aim: Rheumatoid arthritis (RA) is an autoimmune disease and its etiology hasn’t been fully elucidated.
However, excessive amounts of reactive oxygen derivatives have been detected in the synovial fluids of
patients with RA. Therefore, systemic inflammation and oxidative stress are thought to play an
important role in the pathogenesis of RA. Our aim in this study is to investigate the ischemia modified
albumin (IMA), methylglyoxal (MG) levels and serum prolidase activity (SPA) in RA.
Material and Methods: The study were included 50 RA patients and 50 healthy volunteers. IMA levels
and SPA were measured by spectrophotometric method (Perkin Elmer Lambda 25 UV/Vis, US), and MG
levels were measured by chromatographic method (Thermo Ultimate 3000 Ultra-High Performance
Liquid Chromatography). Various hematological and biochemical parameters were determined by
Beckman Coulter LH 780 and AU 5800 (Beckman Coulter, Brea, USA) autoanalyzers, respectively.
Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels were measured by Alifax
(Padova, Italy) and IMMAGE 800 (Beckman Coulter, Brea, USA), respectively.
Results: Serum IMA, MG levels and SPA were found to be significantly higher in the RA group compared
to the control group, while MG and IMA levels of patients with low disease activity (DAS 28 ≤ 3.2) were
found to be statistically significantly lower than patients with moderate disease activity (DAS-28: 3.2-
5.1).
Conclusion: This study shows that oxidative stress and inflammation play an important role in the
pathogenesis of RA, and that there is a relationship between serum IMA and MG levels with disease
activity and duration.
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