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2020, Cilt 18, Sayı 3, Sayfa(lar) 150-156
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Evaluation of κ and λ light chain expressions by flow cytometry analysis in cases of CLL
Çiğdem Özdemir1, Filiz Yavaşoğlu2, Tülay Köken3
1Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi, Tıbbi Patoloji AD., Afyonkarahisar, Türkiye
2Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi, İç Hastalıkları AD, Hematoloji BD, Afyonkarahisar, Türkiye
3Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya AD., Afyonkarahisar, Türkiye
Keywords: Chronic Lymphocytic Leukemia; Flow Cytometry; kappa/lambda

Objective: Confirmation of the clonality of B lymphocytes by flow cytometry analysis has an important role in the diagnosis of Chronic Lymphocytic Leukemia (CLL). In this retrospective study, we investigated which surface light chain pattern they showed as indicative of the monoclonity of CLL cells.

Material and Methods: Flow cytometry results of 33 CLL patients (12 women / 21 men) were examined retrospectively. The reading results of the samples obtained after the application of the antibodies in the CLL immunophenotyping panel and the lysing procedure were evaluated. Cells that co-express CD19 and CD5 were considered CLL cells, and kappa and lambda light chain expressions were evaluated to the monoclonity of these cells.

Results: When all cases were evaluated, the percentage of cases showing kappa monoclonity was 30.3%, while the percentage of cases showing lambda monoclonity was 51.5%. It was observed that both light chain expressions were absent in 18.2% of cases.

Conclusion: In our patients, lambda light chain restriction was observed most frequently in CLL cells.


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