2018, Cilt 16, Sayı 3, Sayfa(lar) 170-176 |
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Evaluation of Serum Immunofixation Electropheresis Data: One-year experience |
Okan Dikker1, Mustafa Şahin2 |
1Sağlık Bilimleri Üniversitesi, Okmeydanı Eğitim ve Araştırma Hastanesi, Tıbbi Biyokimya, İstanbul, Türkiye 2Hitit Üniversitesi, Çorum Erol Olçok Eğitim ve Araştırma Hastanesi, Tıbbi Biyokimya, Çorum, Türkiye |
Keywords: electrophoresis;immunofixation electrophoresis; paraproteinemia |
Aim: Immunofixation electrophoresis (IFE) is a gold standard biochemical technique used in the
diagnosis, treatment and follow-up of monoclonal gammopathies. In paraproteinemias, excessive
production of immunoglobulins (Ig) known as paraprotein or monoclonal protein (M-protein) occurs. Mprotein
can be seen as a sharp band in serum or urine electrophoresis. Paraprotein types produced in
monoclonal gammopathies can be defined by IFE method. In this study, we aimed to retrospectively
evaluate the types of paraproteinemia detected by the IFE method in the laboratory and to compare the
findings with the literature.
Material and Methods: In this study, a total of 403 serum IFE analysis reports performed in the
laboratory were evaluated retrospectively. The laboratory information system was scanned and data of
the patients were obtained. Serum and urine samples of patients were performed by using Interlab G26
analyzer (Interlab Srl, Rome, Italy).
Results: In the study, paraprotein band was detected in 96 patients' serum (23%). IgG kappa (33%) was
detected in 32 patients and IgG lambda (20%) in 19 patients. The presence of paraprotein band was
found to be 23% in patients who underwent urine IFE.
Conclusion: In our study, we found the most IgG kappa and secondly IgG lambda paraproteinemia
types similar to the literature. Our study presented an important projection on the frequency of
monoclonal gammopathy in the laboratory and aimed to contribute to the predictability of the
paraprotein type. Performing serum and urine IFE analysis at the same time will contribute in the
diagnosis of patients with paraprotein band detected in serum protein electrophoresis (SPE) or who are
thought to have paraproteinemia clinically.
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