2015, Cilt 13, Sayı 2, Sayfa(lar) 043-049 |
|
Comparison of BCG and BCP Methods for Albumin Analysis in Hypoalbuminemic Patients |
İdris Mehmetoğlu1, Aysel Kıyıcı2, Sevil Kurban1, Müfide Öncel2 |
1Necmettin Erbakan Üniversitesi Meram Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Konya, Türkiye 2Mevlana Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Konya, Türkiye |
Keywords: serum albumin; bromcresol green; bromcresol purple; intensive care units; hemodialysis |
Background: Bromcresol green (BCG) and Bromcresol Purple (BCP) which are dye-binding based
methods are widely used in serum albumin analysis in clinical laboratories for many years. It was
suggested that discrepancies exist between these methods and these discrepancies are especially more
obvious in hypoalbuminemic patients. We aimed to compare BCG and BCP methods for serum albumin
analysis in patients from intensive care and hemodialysis units.
Material and Methods: Serum samples of 24 patients from hemodialysis and 20 from critical care
units were used in this study. Blood samples received by clinical biochemistry laboratory for routine
controls were firstly analyzed with BCG method. Albumin analysis was repeated with BCP method in the
same samples. Bland Altman graphics and Passing Bablok regression analysis were used to test the
difference between these two methods.
Results: Mean serum albumin concentrations were 3.05±0.60 g/dL and 2.42±0.67 g/dL with BCG and
BCP methods in hemodialysis patients, respectively, while 3.35±0.32 g/dL and 2.55±0.43 g/dL in
patients from critical care unit. Serum albumin concentrations determined with BCG method were
significantly higher than BCP in both groups (p=0.0001 and p=0.0001).
Conclusion: In hypoalbuminemic individuals BCG method overestimates serum albumin concentrations
compared to BCP. This fact should be taken into account in monitoring albumin levels and deciding to
albumin replacement.
|
|
|