2015, Cilt 13, Sayı 1, Sayfa(lar) 021-028 |
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Comparison of Two Different Intact Parathyroid Hormone Assays in Patients with Chronic Kidney Disease |
Buket Kın Tekçe1, Hikmet Tekçe2 |
1Abant İzzet Baysal Üniversitesi Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Bolu, Türkiye 2Abant İzzet Baysal Üniversitesi Tıp Fakültesi, İç Hastalıkları A.D. Nefroloji B.D, Bolu, Türkiye |
Keywords: Kidney diseases; parathyroid hormone |
Objective: Intact parathyroid hormone is used for diagnostic follow up in chronic kidney disease
mineral bone disorder. Studies have shown that there was considerable bias between methods intact
parathyroid hormone assays. This study has investigated the variation between intact parathyroid
hormone assays using Access intact parathyroid hormone and Roche intact parathyroid hormone and if
there is a bias, how this affect clinical decision level.
Materials and Methods: We used blood sample of 41 patients with chronic kidney disease. Intact
parathyroid hormone levels were measured with Access intact parathyroid hormone assay kit through
UniCel DxI 800 autoanalyser (Beckman Coulter, Inc., Fullerton, U.S.A.) and Roche intact parathyroid
hormone assay kit through Cobas e 601 autoanalyser (Roche Diagnostics GmbH, Mannheim, Germany).
The results were divided into groups according to the decision points determined by the manuals.
Results were statistically evaluated.
Results: In Passing-Bablock analyse we found the following equation, y= -9.56 + 0.91 x (confident
interval for intercept: -24.52 to 0.41, for slope: 0.87 to 0.950). There was no significant deviation from
linearity (p>0.05). We found a 16.1 % difference between two methods in Bland–Atman plot analysis.
The moderate agreement was found between clinical decision points determined by two methods using
the Kappa test (κ = 0.437).
Conclusion: We show that there is moderate agreement between Access intact parathyroid hormone
assay and Roche intact parathyroid hormone assay results. We also show that there are differences
between both methods. Clinicians should be informed about the bias between the methods and this
bias should be taken into account in clinical decision point.
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