2014, Cilt 12, Sayı 1, Sayfa(lar) 001-007 |
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Which Method is More Accurate in Patient-side Glucose Monitoring Systems? |
Esra Ekiz1, Şermin Acar Duran2, Mihriban Ulaş2 |
1İstanbul Medeniyet Üniversitesi Göztepe Eğitim Araştırma Hastanesi, İç Hastalıkları, İstanbul, Türkiye 2İstanbul Medeniyet Üniversitesi Göztepe Eğitim Araştırma Hastanesi, Biyokimya, İstanbul, Türkiye |
Keywords: Home blood glucose monitoring; hexokinase; glucose 1-dehydrogenase |
Objective: Comparison of two patient-side glucometers, Accuchek Active (AC) and Optium Xceed
(OP)with autoanalyzer Olympus AU 2700 (OL), which uses the reference method.
Materials and Methods: 44 patients, aged over 18, who attended emergency and internal medicine
clinics at Goztepe Training and Research Hospital, were recruited fort he study. 2 cc blood was drawn
from the antecubital vein and transefered to Standard tubes with EDTA. All samples were studied in
each device twice, mean values were recorded.
Results: Pyrolinquinoline quinon-linked glucose dehydrogenase (GDH) enzyme using bis- (2-
hydroxyethyl)- (4-hydroxyiminohexo 2,5-dieniliden)-ammonium chloride as a mediator, was superior to
NAD (nicotinamide dinucleotide)-linked GDH enzyme using PQ (phenantroline quinone) as a mediator, in measuring glucose levels closer to the reference method. All measurements with hexokinase method
were lower than GDH method and the difference between them showed a linear correlation with
increasing glucose levels.
Conclusion: Accuracy of measurements with regards to the reference method should be the point of
care in choosing patient-side glucometers. It must be kept in mind that all portable glucometers read
higher levels than the reference method and this might be emphasized in education for diabetes.
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